Datasheet

Bcl-rambo Antibody
CATALOG NUMBER: 3023

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 1301 - Human Heart Tissue Lysate
 3) Cat. No. 10-501 - Human Heart Tissue Slide
IMMUNOGEN:Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo.

The immunogen is located within amino acids 280 - 330 of Bcl-rambo.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Bcl-rambo Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bcl-rambo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Bcl-rambo Antibody: MIL1, BCL-RAMBO, Bcl2-L-13, MIL1, CD003, Bcl-2-like protein 13, Bcl-rambo
ACCESSION NO.:AAH07658
PROTEIN GI NO.:14043326
OFFICIAL SYMBOL:BCL2L13
GENE ID:23786

Background

BACKGROUND:Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.
REFERENCES: 1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3) Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4) Kataoka T, Holler N, Michau O, et al. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension. J. Biol. Chem. 2001; 276:19548-54.,

For Research Use Only