Datasheet

Bcl-G Antibody
CATALOG NUMBER: 3165

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (92%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1215 - U937 Cell Lysate
 2) Cat. No. 1416 - Mouse Testis Tissue Lysate
SPECIFICITY:Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
IMMUNOGEN:Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-G.

The immunogen is located within the first 50 amino acids of Bcl-G.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Bcl-G Antibody is Ion exchange chromatography purified.
PHYSICAL STATE:Liquid
BUFFER:Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bcl-G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Bcl-G Antibody: BCLG, BCLG, Apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14
ACCESSION NO.:NM_030766
PROTEIN GI NO.:13540528
OFFICIAL SYMBOL:BCL2L14
GENE ID:79370

Background

BACKGROUND:Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.
REFERENCES: 1) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
2) Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
3) Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.

For Research Use Only