Datasheet

Bcl-B Antibody
CATALOG NUMBER: 3163

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 22 kDa

Observed: 26 kDa
IMMUNOGEN:Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B.

The immunogen is located within amino acids 90 - 140 of Bcl-B.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Bcl-B Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Bcl-B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bcl-B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Bcl-B Antibody: Boo, Diva, BCL-B, BCLB, Bcl-2-like protein 10, Anti-apoptotic protein NrH, Bcl2-L-10
ACCESSION NO.:NP_065129
PROTEIN GI NO.:9966783
OFFICIAL SYMBOL:BCL2L10
GENE ID:10017

Background

BACKGROUND:Bcl-B Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.
REFERENCES: 1) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607
2) Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
3) Ke N, Godzik A, and Reed JC. Bcl-B: A novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J. Biol. Chem. 2001; 276:12481-4.
4) Song Q, Kuang Y, Dixit VM, et al. Boo, a negative regulator of cell death, interacts with Apaf-1. EMBO J. 1999; 18:167-78.

For Research Use Only