Bcl-2 Antibody Cat. No.: 3337

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (93%), Chicken: (86%)
IMMUNOGEN: Anti-Bcl-2 antibody (3337) was raised against a peptide corresponding to 14 amino acids near the center of human Bcl-2.

The immunogen is located within amino acids 80-130 of Bcl-2.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: WB: 1-4μg/mL; ICC: 2 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 1205 - Jurkat Cell Lysate
 3) Cat. No. 1208 - THP-1 Cell Lysate
 4) Cat. No. 1305 - Human Kidney Tissue Lysate
 5) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 26kD

Observed: 26 kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain.  Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD). Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD). Rat Bcl-2 has one isoform (236aa, 27kD). 3337 can detect all isoforms of human, mouse and rat

psi-iconProperties
PURIFICATION:Bcl-2 Antibody is Ion exchange chromatography purified.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bcl-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BCL2
ALTERNATE NAMES:Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2
ACCESSION NO.:AAH27258
PROTEIN GI NO.:20072668
GENE ID:596
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
REFERENCES:1) Lockshin et al. Cell Death Differ. 2000; 7:2-7.
2) Cory et al. Oncogene 2003; 22:8590-607.
3) Heiser et al. Exp. Geron. 2004; 39:1125-35.
4) Hockenbery et al. Nature 1990; 348:334-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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