| HOST SPECIES: | Mouse |
| SPECIES REACTIVITY: | Human |
| IMMUNOGEN: | A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody. |
| TESTED APPLICATIONS: | ELISA, Flow, IF, IHC, WB |
| APPLICATIONS: | ELISA: order BSA-free format for coating Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml WB: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. |
| SPECIFICITY: | Does not react with mouse and rat |
Specifications| PURIFICATION: | Protein G affinity chromatography |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG1, kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| OFFICIAL SYMBOL: | BCL2 |
| ALTERNATE NAMES: | Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2 |
| GENE ID: | 596 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative. |
ANTIBODIES FOR RESEARCH USE ONLY.
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