Datasheet

BCAS4 Antibody
CATALOG NUMBER: 5631

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BCAS4 antibody can be used for detection of BCAS4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
IMMUNOGEN:BCAS4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human BCAS4.

The immunogen is located within the first 50 amino acids of BCAS4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BCAS4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BCAS4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BCAS4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BCAS4 Antibody: CNOL, Breast carcinoma-amplified sequence 4
ACCESSION NO.:CAI23006
PROTEIN GI NO.:56203146
OFFICIAL SYMBOL:BCAS4
GENE ID:55653

Background

BACKGROUND:BCAS4 Antibody: The Breast carcinoma amplified sequence (BCAS4) gene is localized to 20q13, a region that is often amplified in breast cancers. It was isolated by the cloning of a highly overexpressed 1.3 kb transcript from the fusion of the last two exons of the BCAS3 and BCAS4 genes. This BCAS3-BCAS4 fusion transcript is only found in the MCF7 cell line, but the BCAS4 gene is also overexpressed in nine of thirteen breast cancer cell lines. The role of BCAS4 is unknown but its mRNA is highly expressed in thymus, spleen, kidney, brain, and placenta, suggesting BCAS4 plays other roles in multiple tissues.
REFERENCES: 1) Barlund M, Monni O, Weaver JD, et al. Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer. Genes Chromosomes Cancer2002; 35:311-7.

For Research Use Only