Datasheet

BCAS3 Antibody
CATALOG NUMBER: 5629

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BCAS3 antibody can be used for detection of BCAS3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
IMMUNOGEN:BCAS3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human BCAS3.

The immunogen is located within amino acids 50 - 100 of BCAS3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BCAS3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BCAS3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BCAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BCAS3 Antibody: MAAB, GAOB1, Breast carcinoma-amplified sequence 3
ACCESSION NO.:EAW51415
PROTEIN GI NO.:119571800
OFFICIAL SYMBOL:BCAS3
GENE ID:54828

Background

BACKGROUND:BCAS3 Antibody: The Breast carcinoma amplified sequence (BCAS3) gene is localized to 17q23, a region that is often amplified in breast cancers. Copy number gains at this region have also been reported in other tumors such as brain, lung, liver, testis and bladder. BCAS3 is thought to be an estrogen receptor (ER)-alpha co-activator that acts through PELP1, another ER-alpha that in turn activates BCAS3 expression. This may set up a positive feedback loop leading to ER-a signal amplification in the cell which may play a significant role in breast cancer. BCAS3 expression has also been observed in embryonic stem cells and vascular precursors, suggesting that it may also play a role in embryogenesis and tumor angiogenesis.
REFERENCES: 1) Barlund M, Monni O, Weaver JD, et al. Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer. Genes Chromosomes Cancer2002; 35:311-7.
2) Sinclair CS, Rowley M, Naderi A, et al. The 17q23 amplicon and breast cancer. Breast Cancer Res. Treat.2003; 78:313-22.
3) Gururaj AE, Peng S, Vadlamudi RK, et al. Estrogen induces expression of BCAS3, a novel estrogen receptor-a coactivator, through Proline-, Glutamic acid-, and leucine-rich protein -1 (PELP1). Mol. Endocrinol.2007; 21:1847-60.
4) Siva K, Venu P, Mahadevan A, et al. Human BCAS3 expression in embryonic stem cells and vascular precursors suggests a role,in human ebryogenesis and tumor angiogenesis. PloS One2007; 11:e1202.

For Research Use Only