Datasheet

BCAS1 Antibody
CATALOG NUMBER: 5625

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (75%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BCAS1 antibody can be used for detection of BCAS1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
IMMUNOGEN:BCAS1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human BCAS1.

The immunogen is located within amino acids 460 - 510 of BCAS1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BCAS1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BCAS1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BCAS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BCAS1 Antibody: AIBC1, NABC1, AIBC1, Breast carcinoma-amplified sequence 1, Amplified and overexpressed in breast cancer
ACCESSION NO.:EAW75575
PROTEIN GI NO.:119595981
OFFICIAL SYMBOL:BCAS1
GENE ID:8537

Background

BACKGROUND:BCAS1 Antibody: BCAS1 was identified through positional cloning and was found to be overexpressed in most but not all breast cancer cells lines. Little is known about this protein; BCAS1 is cytoplasmically localized and is thought to form homodimers through coiled-coil structures. High levels of BCAS1 mRNA are seen in brain and prostate tissues, with lower amounts observed in colon, intestine and testis. Cells engineered to overexpress BCAS1 did not lose anchorage-dependent growth or increase their rate of growth, suggesting that BCAS1 is not a prototypical oncogene. The BCAS1 gene was also found to be amplified in other carcinomas such as pancreatic carcinoma, suggesting that BCAS1 may play in important role in the control of cell proliferation.
REFERENCES: 1) Collins C, Rommens JM, Kowbel D, et al. Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. Proc. Natl. Acad. Sci. USA1998; 95:8703-8.
2) Beardsley DI, Kowbel D, Lataxes TA, et al. Characterization of the novel amplified in breast cancer-1 (NABC1) gene product. Exp. Cell Res.2003; 290:402-13.
3) Loukopoulos P, Shibata T, Katoh H, et al. Genome-wide assay-based comparative genomic hybridization analysis of pancreatic adenocarcinoma: identification of genetic indicators that predict patient outcome. Cancer Sci.2007; 98:392-400.

For Research Use Only