Bax Antibody [ARBX-1] Cat. No.: 33-964

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: Recombinant full-length human protein was used as the immunogen for the Bax antibody.
TESTED APPLICATIONS: IF, IHC-P, WB
APPLICATIONS: Immunofluorescence: 1-2 ug/ml

Western blot: 1-2 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Bax antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

SPECIFICITY: Does not react with mouse and rat

psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:BAX
ALTERNATE NAMES:Bax Antibody: BCL2L4, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
GENE ID:581
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:BCL2 associated X protein/Bax accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt]

ANTIBODIES FOR RESEARCH USE ONLY.

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