Datasheet

BATF Antibody
CATALOG NUMBER: 7069

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1466 - Rat Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT:14 kDa
SPECIFICITY:Multiple isoforms of BATF are known to exist. Despite its predicted molecular weight, BATF often migrates at a higher than expected molecular weight.
IMMUNOGEN:BATF antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BATF.

The immunogen is located within the first 50 amino acids of BATF.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BATF Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BATF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BATF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BATF Antibody: SFA2, B-ATF, BATF1, SFA-2, Basic leucine zipper transcriptional factor ATF-like, B-cell-activating transcription factor
ACCESSION NO.:NP_006390
PROTEIN GI NO.:5453563
OFFICIAL SYMBOL:BATF
GENE ID:10538

Background

BACKGROUND:BATF Antibody: BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.
REFERENCES: 1) Dorsey MJ, Tae HJ, Sollenberger KG, et al. B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family. Oncogene 1995; 11:2255-65.
2) Echlin DR, Tae HJ, Mitin N, et al. B-ATF functions as a negative regulator of AP-1 mediated transcription and blocks cellular transformation by Ras and Fos. Oncogene 2000; 19:1752-63.
3) Schraml BU, Hildner K, Ise W, et al. The AP-1 transcription factor Batf controls TH17 differentiation. Nature 2009; 460:405-9.
4) Ise W, Kohyama M, Schraml BU, et al. The transcription factor BATF controls the global regulators of class-switch recombination in both B cells and T cells. Nat. Immunol. 2011; 12:536-43.

For Research Use Only