BAPX1 Antibody Cat. No.: 6211

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
IMMUNOGEN: BAPX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human BAPX1.

The immunogen is located within amino acids 180 - 230 of BAPX1.
TESTED APPLICATIONS: IF, IHC-P
APPLICATIONS: BAPX1 antibody can be used for detection of BAPX1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: BAPX1 antibody is predicted not to cross-react with other NKX homeobox proteins.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate

psi-iconProperties
PURIFICATION:BAPX1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BAPX1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAPX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:NKX3-2
ALTERNATE NAMES:BAPX1 Antibody: SMMD, BAPX1, NKX3B, NKX3.2, Bagpipe homeobox protein homolog 1
ACCESSION NO.:NP_001180
PROTEIN GI NO.:4502365
GENE ID:579
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BAPX1 Antibody: BAPX1 is the mammalian homolog of the Drosophila bagpipe homeobox gene and is expressed in the splanchnic mesoderm and embryonic skeleton. It is one of the earliest developmental markers for the sclerotome portion of the somite and the gut mesentery. BAPX1 is required for normal skeletal development; homozygous inactivating mutations in the BAPX1 gene result in spodylo-megaepiphyseal-metaphyseal dysplasia (SMMD). It has also been suggested to play a role in the proper development of the mammalian gut and is required for distal stomach development as part of a BARX1-dependent pathway.
REFERENCES:1) Tribioli C, Frasch M, and Lufkin T. Bapx1: an evolutionary conserved homologue of the Drosophila bagpipe homeobox gene is expressed in splanchnic mesoderm and the embryonic skeleton. Mech. Dev. 1997; 65:145-62.
2) Hellemans J, Simon M, Dheedene A, et al. Homozygous inactivating mutations in the NKX3-2 gene result in spodylo-megaepiphyseal-metaphyseal dysplasia. Am. J. Hum. Genet. 2009; 85:916-22.
3) Verzi MP, Stanfel MN, Moses KA, et al. Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development. Gastroenterology 2009; 136:1701-10.

ANTIBODIES FOR RESEARCH USE ONLY.

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