Datasheet

BAPX1 Antibody
CATALOG NUMBER: 6211

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BAPX1 antibody can be used for detection of BAPX1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
SPECIFICITY:BAPX1 antibody is predicted not to cross-react with other NKX homeobox proteins.
IMMUNOGEN:BAPX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human BAPX1.

The immunogen is located within amino acids 180 - 230 of BAPX1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BAPX1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BAPX1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAPX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BAPX1 Antibody: SMMD, BAPX1, NKX3B, NKX3.2, Bagpipe homeobox protein homolog 1
ACCESSION NO.:NP_001180
PROTEIN GI NO.:4502365
OFFICIAL SYMBOL:NKX3-2
GENE ID:579

Background

BACKGROUND:BAPX1 Antibody: BAPX1 is the mammalian homolog of the Drosophila bagpipe homeobox gene and is expressed in the splanchnic mesoderm and embryonic skeleton. It is one of the earliest developmental markers for the sclerotome portion of the somite and the gut mesentery. BAPX1 is required for normal skeletal development; homozygous inactivating mutations in the BAPX1 gene result in spodylo-megaepiphyseal-metaphyseal dysplasia (SMMD). It has also been suggested to play a role in the proper development of the mammalian gut and is required for distal stomach development as part of a BARX1-dependent pathway.
REFERENCES: 1) Tribioli C, Frasch M, and Lufkin T. Bapx1: an evolutionary conserved homologue of the Drosophila bagpipe homeobox gene is expressed in splanchnic mesoderm and the embryonic skeleton. Mech. Dev. 1997; 65:145-62.
2) Hellemans J, Simon M, Dheedene A, et al. Homozygous inactivating mutations in the NKX3-2 gene result in spodylo-megaepiphyseal-metaphyseal dysplasia. Am. J. Hum. Genet. 2009; 85:916-22.
3) Verzi MP, Stanfel MN, Moses KA, et al. Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development. Gastroenterology 2009; 136:1701-10.

For Research Use Only