BAP31 Antibody Cat. No.: 3675

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-BAP31 antibody (3675) was raised against a peptide corresponding to 16 amino acids near the center of human BAP31.

The immunogen is located within amino acids 130 - 180 of BAP31.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: WB: 0.5-2 μg/mL; IHC: 10 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1462 - Rat Lung Tissue Lysate
 2) Cat. No. 1204 - K562 Cell Lysate
 3) Cat. No. 1223 - Caco-2 Cell Lysate
 4) Cat. No. 1282 - 3T3/NIH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 28 or 34kD

Observed: 28 kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar BAP31 expression profile in human cell lines detected by two independent anti-BAP31 antibodies that recognize different epitopes, 3675 against central domain and 64-237, against recombinant protein.  BAP31 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies.

ISOFORMS: Human BAP31 has 2 isoforms, including isoform 1 (246aa, 28kD) and isoform 2 (313aa, 35kD). Mouse BAP31 has one isoform (245aa, 28kD) and Rat BAP31 also has one isoform (245aa, 28kD). 3675 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:BAP31 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAP31 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BCAP31
ALTERNATE NAMES:BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31
ACCESSION NO.:NP_005736
PROTEIN GI NO.:32171186
GENE ID:10134
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. Bap31 may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.
REFERENCES:1) Ng et al. J. Cell Biol.1997; 139:327-38.
2) Kim et al. EMBO J.1994; 13:3793-800.
3) Zen et al. J. Biol. Chem.2004; 279:44924-30.

ANTIBODIES FOR RESEARCH USE ONLY.

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