Datasheet

BAP31 Antibody
CATALOG NUMBER: 3665

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:BAP31 antibody can be used for the detection of BAP31 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1225 - Ramos Cell Lysate
 2) Cat. No. 1462 - Rat Lung Tissue Lysate
IMMUNOGEN:BAP31 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human BAP31.

The immunogen is located within the last 50 amino acids of BAP31.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BAP31 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAP31 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31
ACCESSION NO.:NP_005736
PROTEIN GI NO.:32171186
OFFICIAL SYMBOL:BCAP31
GENE ID:10134

Background

BACKGROUND:BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. It may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopicially, is also a potent inducer cell death.
REFERENCES: 1) Ng F, Nguyen M, Kwan T, et al. p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated proteinin the endoplasmic reticulum. J. Cell Biol.1997; 139:327-38.
2) Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J.1994; 13:3793-800.
3) Paquet M-E, Cohen-Doyle M, Shore GC, et al. Bap29/31 influences the intracellular traffic of MHC class I molecules. J. Immunol.2004; 172:7548-55.
4) Zen K, Utech M, Liu Y, et al. Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils. J. Biol. Chem.2004; 279:44924-30.

For Research Use Only