Datasheet

BAP29 Antibody
CATALOG NUMBER: 3667

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (88%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Bap29 antibody can be used for the detection of Bap29 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
IMMUNOGEN:BAP29 antibody was raised against a 17 amino acid synthetic peptide from near the center of human Bap29 Bap29 antibody is specific for isoform a of Bap29.

The immunogen is located within amino acids 130 - 180 of BAP29.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BAP29 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BAP29 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAP29 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BAP29 Antibody: BAP29, BAP29, B-cell receptor-associated protein 29, BCR-associated protein 29
ACCESSION NO.:NP_001008405
PROTEIN GI NO.:56549093
OFFICIAL SYMBOL:BCAP29
GENE ID:55973

Background

BACKGROUND:BAP29 Antibody: Bap29 and the related protein Bap31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both Bap29 and Bap31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the Bap29/Bap31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that Bap29 and Bap31 may act as chaperones transmembrane regions of various proteins. Bap29 possesses multiple isoforms.
REFERENCES: 1) Kim KM, Adachi T, Nielsen PJ, et al. Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J.1994; 13:3793-800.
2) Ng F, Nguyen M, Kwan T, et al. p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein the endoplasmic reticulum. J. Cell Biol.1997; 139:327-38.
3) Schamel WW, Kuppig S, Becker B, et al. A high-molecular-weight complex of membrane proteins BAP29/BAP31 is involved in the retention of membrane-bound IgD in the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA2003; 100:9861-6.

For Research Use Only