Datasheet

BANP Antibody
CATALOG NUMBER: 5977

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BANP antibody can be used for detection of BANP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
IMMUNOGEN:BANP antibody was raised against a 14 amino acid synthetic peptide near the center of human BANP.

The immunogen is located within amino acids 300 - 350 of BANP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BANP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BANP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BANP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BANP Antibody: BEND1, SMAR1, SMARBP1, BEND1, Protein BANP, BEN domain-containing protein 1
ACCESSION NO.:NP_001167014
PROTEIN GI NO.:291084803
OFFICIAL SYMBOL:BANP
GENE ID:54971

Background

BACKGROUND:BANP Antibody: BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-beta signaling. Furthermore, BANP can modulate NF-κB transactivation and can inhibit tumorigenesis by regulating NF-κB target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix.
REFERENCES: 1) Birot A, Duret L, Bartholin L, et al. Identification and molecular analysis of BANP. Gene2000; 253:189-96.
2) Kaul R, Mukherjee S, Ahmed F, et al. Direct interaction with and activation of p53 by SMAR1 retards cell-cycle progression at G2/M phase and delays tumor growth in mice. Int. J. Cancer2003; 103:606-15.
3) Singh K, Mogare D, Giridharagopalan RO, et al. P%3 target gene SMAR1 is dysregulated in breast cancer: its role in cancer cell migration and invasion. PLoS One2007; 2:e660.
4) Signh K, Sinha S, Malonia SK, et al. Tumor suppressor SMAR1 repsses IkBa expression and inhibits p65 transactivation through matrix attachment regions. J. Biol. Chem.2009; 284:1267-78.

For Research Use Only