BANF1 Antibody Cat. No.: 4019

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: Anti-BANF1 antibody (4019) was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1.

The immunogen is located within the first 50 amino acids of BANF1.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: WB: 1-2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 10kD

Observed: 12 kD

Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar BANF1 expression profile in human and mouse cell lines detected by two independent anti-BANF1 antibodies that recognize different epitopes, 4017 against C-terminus domain and 4019 against N-terminus domain. BANF1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

ISOFORMS: Human BANF1 has one isoform (89aa, 10kD). Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD). 4019 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:BANF1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BANF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:BANF1
ALTERNATE NAMES:BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1
ACCESSION NO.:AAH05942
PROTEIN GI NO.:13543577
GENE ID:8815
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.
REFERENCES:1) Furukawa. J. Cell Sci. 1999; 112:2485-92.
2) Cai et al. EMBO J. 2001; 20:4399-407.
3) Chen and Engelman. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.

ANTIBODIES FOR RESEARCH USE ONLY.

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