Datasheet

BAMBI Antibody
CATALOG NUMBER: 7931

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Sheep: (88%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1402 - Mouse Lung Tissue Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 29 kDa

Observed: 26 kDa
SPECIFICITY:BAMBI antibody is human, mouse and rat reactive.
IMMUNOGEN:BAMBI antibody was raised against a 17 amino acid peptide near the center of human BAMBI.

The immunogen is located within amino acids 100 - 150 of BAMBI.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BAMBI antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BAMBI antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAMBI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BMP and activin membrane-bound inhibitor homolog
ACCESSION NO.:NP_036474
PROTEIN GI NO.:6912534
OFFICIAL SYMBOL:BAMBI
GENE ID:25805

Background

BACKGROUND:BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).
REFERENCES: 1) Onichtchouk D, Chen YG, Dosch R, et al. Silencing of TGF-beta signalling by the pseudoreceptor BAMBI. Nature 1999; 401:480-5.
2) Yan X, Lin Z, Chen F, et al. Human BAMBI cooperates with Smad7 to inhibit transforming growth factor-beta signaling. J Biol. Chem. 2009; 284:30097-104.
3) Grotewold L, Plum M, Dildrop R, et al. Bambi is coexpressed with Bmp-4 during mouse embryogenesis. Mech. Dev. 2001; 100:327-30.
4) Fritzmann J, Morkel M, Besser D, et al. A colorectal cancer expression profile that includes transforming growth factor beta inhibitor BAMBI predicts metastatic potential. Gastroenterology 2009; 137:165-75.

For Research Use Only