Datasheet

Bak Antibody
CATALOG NUMBER: 3347

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:Bak antibody can be used for detection of Bak by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1284 - L1210 Cell Lysate
 2) Cat. No. 17-204 - L1210 Cell Slide
IMMUNOGEN:Bak antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak.

The immunogen is located within amino acids 30 - 80 of Bak.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Bak Antibody is Ion exchange chromatography purified.
PHYSICAL STATE:Liquid
BUFFER:Bak Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Bak antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Bak Antibody: BAK, CDN1, BCL2L7, BAK-LIKE, BAK, Apoptosis regulator BAK, Bcl2-L-7
ACCESSION NO.:Q16611
PROTEIN GI NO.:2493274
OFFICIAL SYMBOL:BAK1
GENE ID:578

Background

BACKGROUND:Bak Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria. It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability.
REFERENCES: 1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3) Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4) Kiefer M, Brauer MJ, Powers VC, et al. Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. Nature 1995; 374:736-9.

For Research Use Only