BAFF Antibody [2B] Cat. No.: XP-5728-M

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: The monoclonal antibody was produced in BALB/c mice using recombinant human BAFF as the immunizing antigen.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 5.0-6.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human BAFF when used with our biotinylated antigen affinity purified anti-human BAFF (XP-5105Bt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL.

Western Blot:

To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 2.0-4.0 ng/lane, under non-reducing conditions.

psi-iconProperties
PURIFICATION:This IgG1/K antibody was purified from ascites fluid on a Protein A chromatography column.
CLONALITY:Monoclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:BAFF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

psi-iconAdditional Info
OFFICIAL SYMBOL:TNFSF13B
ALTERNATE NAMES:B cell Activating Factor , BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, DTL, CD257, THANK, TALL-1
ACCESSION NO.:Q9Y275
PROTEIN GI NO.:13124573
GENE ID:10673
USER NOTE:Centrifuge vial prior to opening.

ANTIBODIES FOR RESEARCH USE ONLY.

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