BAFF Antibody Cat. No.: 2221

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-BAFF antibody (2221) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF.

The immunogen is located within the last 50 amino acids of BAFF.
TESTED APPLICATIONS: ELISA, ICC, IF, IHC, WB
APPLICATIONS: WB: 0.25-1 μg/mL; ICC: 1 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1209 - HL60 Cell Lysate
 2) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 3) Cat. No. 17-009 - HL-60 Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 31kD

Observed: 35 kD
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 3): Anti-BAFF antibodies (2221) detected human BAFF recombinant protein at different concentrations.

Regulated expression validation (Figure 6-8): BAFF expression detected by anit-BAFF antibodies (2221) was up-regulated  in patients with myloma or Graves’ disease or in murine transplanted heart.

ISOFORMS: Human BAFF has 3 isoforms, including isoform 1 (285aa, 31kD), isoform 2 (266aa, 29kD) and isoform 3 (164aa, 17.6kD). Mouse BAFF has 2 isoforms, including isoform 1 (309aa, 34kD) and isoform 2 (290aa, 32kD). Rat BAFF has only one isoform (274aa, 30kD). 2221 can detect two longer human isoforms, mouse and rat.

psi-iconProperties
PURIFICATION:BAFF Antibody is Ion exchange chromatography purified.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:BAFF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAFF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:TNFSF13B
ALTERNATE NAMES:BAFF Antibody: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator
ACCESSION NO.:NP_006564
PROTEIN GI NO.:5730097
GENE ID:10673
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.
REFERENCES:1) Moore et al. Science 1999;285:260-3.
2) Schneider et al. J Exp Med 1999;189:1747-56.
3) Shu et al. J Leukoc Biol 1999;65:680-3.
4) Mukhopadhyay et al. J Biol Chem 1999 ;274:15978-81.
psi-iconCITATIONS
CITATIONS: 1)Tai et al. Role of B-cell-activating factor in adhesion and growth of human multiple myeloma cells in the bone marrow microenvironment. Cancer Res. 2006;66(13):6675-82. PMID: 16818641
2)Campi et al. B Cell Activating Factor (BAFF) and BAFF Receptor Expression in Autoimmune and Nonautoimmune Thyroid Diseases. Thyroid. 2015;25(9):1043-9. PMID: 26214745
3)Ye et al. BAFF binding to T cell-expressed BAFF-R costimulates T cell proliferation and alloresponses. Eur J Immunol. 2004;34(10):2750-9. PMID: 15368291
4)Gavin et al. DeltaBAFF, an alternate splice isoform that regulates receptor binding and biopresentation of the B cell survival cytokine, BAFF. J Biol Chem. 2003;278(40):38220-8. PMID: 12867412

ANTIBODIES FOR RESEARCH USE ONLY.

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