BAD (phospho Ser136) Antibody Cat. No.: 79-135

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Mouse
IMMUNOGEN: BAD (Phospho-Ser136) antibody was raised against a peptide sequence around phosphorylation site of serine136 (S-R-S (p) -A-P) derived from Mouse BAD.
TESTED APPLICATIONS: IHC, WB
APPLICATIONS: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
SPECIFICITY: This antibody detects endogenous level of BAD only when phosphorylated at serine136.
PREDICTED MOLECULAR WEIGHT: 23 kDa

psi-iconProperties
PURIFICATION:Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Store antibody at -20˚C for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:BAD
ALTERNATE NAMES:BBC2, BCL2L8, BBC6
ACCESSION NO.:NP_004313.1
PROTEIN GI NO.:10835069
GENE ID:572
psi-iconBackground and References
BACKGROUND:The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
REFERENCES:1) Wang XQ, et al. (2001) . J Biol Chem. 276 (48): 44504-44511. Lee YI, et al. (2001) . J Biol Chem. 276 (20): 16969-16977. Maiti D, et al. (2001) . J Biol Chem. 276 (1): 329-333.

ANTIBODIES FOR RESEARCH USE ONLY.

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