| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | BACE2 antibody was raised against a 16 amino acid peptide near the amino terminus of human BACE2. The immunogen is located within amino acids 30 - 80 of BACE2. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | BACE2 can be used for detection of BACE2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. Antibody validated: Western Blot in human and mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
| 2) Cat. No. 1401 - Mouse Heart Tissue Lysate | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 57 kDa Observed: 66 kDa |
Specifications| PURIFICATION: | BACE2 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | BACE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | BACE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | BACE2 |
| ALTERNATE NAMES: | BACE2 Antibody: ASP1, BAE2, DRAP, AEPLC, ALP56, ASP21, CDA13, CEAP1, UNQ418/PRO852, Beta-secretase 2, Aspartic-like protease 56 kDa, ASP1 |
| ACCESSION NO.: | AAF17078 |
| PROTEIN GI NO.: | 6561812 |
| GENE ID: | 25825 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | BACE2 Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1. BACE2 also cleaves APP at beta-site and at a different site within Abeta. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region', suggesting that BACE2 and Abeta may also contribute to the pathogenesis of Down syndrome. |
| REFERENCES: | 1) Vassar R, et al. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999; 286:735-41. | 2) Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as β-secretase. Mol Cell Neurosci 1999; 14:419-27. | 3) Sinha S, et al. Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 1999; 402:537-40. | 4) Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. Nature 1999; 402:533-7. |
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