Specifications
Properties
Additional Info
Background
Specifications
| SPECIES REACTIVITY: | Human, Mouse |
|---|---|
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Guinea pig: (94%), Rat: (94%), Bovine: (94%) |
| TESTED APPLICATIONS: | ELISA, ICC, IF, IHC-P, WB |
| APPLICATIONS: | BACE can be used for detection of BACE by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| POSITIVE CONTROL: | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
| 2) Cat. No. 1212 - 3T3 Cell Lysate | |
| 3) Cat. No. 17-201 - 3T3/BALB Cell Slide | |
| PREDICTED MOLECULAR WEIGHT: | 70 kDa |
| IMMUNOGEN: | BACE antibody was raised against a peptide corresponding to 17 amino acids at the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE. |
| HOST SPECIES: | Rabbit |
Properties
| PURIFICATION: | BACE Antibody is Ion exchange chromatography purified. |
|---|---|
| PHYSICAL STATE: | Liquid |
| BUFFER: | BACE Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | BACE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
Additional Info
| ALTERNATE NAMES: | BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2 |
|---|---|
| ACCESSION NO.: | AF190725 |
| PROTEIN GI NO.: | 6118538 |
| OFFICIAL SYMBOL: | BACE1 |
| GENE ID: | 23621 |
Background
| BACKGROUND: | BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP. |
|---|---|
| REFERENCES: | 1) Vassar R, Bennett BD, Babu-Khan S, et al. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41 | 2) Hussain I, Powell D, Howlett DR , et al. Identification of a novel aspartic protease (Asp 2) as β-secretase. Mol Cell Neurosci 1999;14:419-27 | 3) Yan R, Bienkowski MJ, Shuck ME, et al. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. Nature 1999;402:533-7 | 4) Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 1999;402:537-40 (WD0500) |
For Research Use Only
