The extracellular domain of mouse B7-H4 (aa 29-258) is fused to the N-terminus of the Fc region of mouse IgG2a.
FUSION TAG:
Fc Tag
TESTED APPLICATIONS:
APPLICATIONS:
This recombinant proteins is for research use only.
BIOLOGICAL ACTIVITY:
Measured by its ablility to inhibit anti-CD3-induced proliferation of stimulated mouse T cells.
Properties
PURITY:
≥98% (SDS-PAGE).
Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
PHYSICAL STATE:
Lyophilized
BUFFER:
Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration.
STORAGE CONDITIONS:
Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.
Additional Info
ALTERNATE NAMES:
V-set Domain-containing T Cell Activation Inhibitor 1, VTCN1, B7h.5, Immune Costimulatory Protein B7-H4, T Cell Costimulatory Molecule B7x, Protein B7S1
ACCESSION NO.:
NP_848709
PROTEIN GI NO.:
260166624
OFFICIAL SYMBOL:
Vtcn1
GENE ID:
242122
Background and References
BACKGROUND:
B7-H4 is a B7 family member that negatively regulates T cell immunity by inhibiting T cell proliferation, cytokine production and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production and generation of alloreactive cytotoxic T cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.
Specifications
