B7H3 Antibody [10G6] Cat. No.: RF16095

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: B7-H3 antibody was raised against the extracellular domain of human B7-H3
TESTED APPLICATIONS: ELISA, Flow, ICC, IF, IHC-P
APPLICATIONS: B7-H3 antibody can be used for detection of B7-H3 for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide

psi-iconProperties
PURIFICATION:B7-H3 Antibody is supplied as protein A purified IgG1.
CLONALITY:Monoclonal
ISOTYPE:IgG1,k
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:B7-H3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD276
ALTERNATE NAMES:B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276
ACCESSION NO.:NP_001019907
PROTEIN GI NO.:67188443
GENE ID:80381
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
REFERENCES:1) Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74.
2) Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7.
3) Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51.
4) Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe?. Int J Cancer 2014; 134:2764-71.

ANTIBODIES FOR RESEARCH USE ONLY.

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