This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY:
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-1, Fc Tag with a linear range of 1-8 ng/mL (QC tested).
Properties
PURITY:
>95% as determined by reduced SDS-PAGE.
Less than 1.0 EU per μg by the LAL method.
PREDICTED MOLECULAR WEIGHT:
50.5 kDa
PHYSICAL STATE:
Lyophilized
BUFFER:
Tris with Glycine, Arginine and NaCl, pH7.5
STORAGE CONDITIONS:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Additional Info
ALTERNATE NAMES:
CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
ACCESSION NO.:
G7NXN7
OFFICIAL SYMBOL:
B7-1
GENE ID:
102140078
Background and References
BACKGROUND:
B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-kappaB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
REFERENCES:
1) Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7.
Specifications
