| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Mouse: (83%), Rat: (83%) |
| IMMUNOGEN: | B2M antibody was raised against a 15 amino acid peptide near the carboxy terminus of human B2M. The immunogen is located within the last 50 amino acids of B2M. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | B2M antibody can be used for detection of B2M by Western blot at 1 - 2 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
| SPECIFICITY: | B2M antibody is human reactive. |
| POSITIVE CONTROL: | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 13 kDa Observed: 12 kDa |
Specifications| PURIFICATION: | B2M Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | B2M Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | B2M antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | B2M |
| ALTERNATE NAMES: | B2M Antibody: Beta-2-microglobulin |
| ACCESSION NO.: | NP_004039 |
| PROTEIN GI NO.: | 4757826 |
| GENE ID: | 567 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | B2M Antibody: Beta2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). B2M is involved in the presentation of peptide antigens to the immune system and plays a critically important role in immune system function (3). It is expressed on nearly all nucleated cells and contains one Ig-like C1-type (immunoglobulin-like) domain (2,3). Mutations in the Beta 2-microglobulin gene can enhance the progression of malignant melanoma and osteoarthropathy (4,5). |
| REFERENCES: | 1) Krangel MS, Orr HT, and Strominger JL. Assembly and maturation of HLA-A and HLA-B antigens in vivo. Cell 1979; 18:979-91. | 2) Skjodt K, Welinder KG, Crone M, et al. Isolation and characterization of chicken and turkey beta 2-microglobulin. Mol. Immunol. 1986; 23:1301-9. | 3) Ohashi K. Pathogenesis of beta2-microglobulin amyloidosis. Pathol. Int. 2001; 51:1-10. | 4) Blum C, Graham A, Yousefzadeh M, et al. The expression ratio of Map7/B2M is prognostic for survival in patients with stage II colon cancer. Int. J. Oncol. 2008; 33:579-84. |
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