B-raf Antibody Cat. No.: 7379

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%)
IMMUNOGEN: Anti-B-raf antibody (7379) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf.

The immunogen is located within amino acids 330 - 380 of B-raf.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-2 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: This antibody will not cross-react with C-raf.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
 3) Cat. No. 1224 – Daudi Cell Lysate
 4) Cat. No. 1204 – K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 94kD

Observed: 94 kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain.  B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf a has one isoform (712aa, 80kD). 7379 can detect in human B-raf and mouse isoform 1.

psi-iconProperties
PURIFICATION:B-raf Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:B-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:BRAF
ALTERNATE NAMES:B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
ACCESSION NO.:P15056
PROTEIN GI NO.:50403720
GENE ID:673
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
REFERENCES:1) McCubrey et al. Biophys. Acta 2007; 1773:1263-84.
2) Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.
3) Sarkozy et al. Hum. Mutat.2009; 30:695-702.

ANTIBODIES FOR RESEARCH USE ONLY.

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