| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Chicken: (85%) |
| IMMUNOGEN: | AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. The immunogen is located within amino acids 780 - 830 of AXIN2. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. |
| SPECIFICITY: | AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four. |
| POSITIVE CONTROL: | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | 85, 93 kDa |
Specifications| PURIFICATION: | AXIN2 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | AXIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | AXIN2 |
| ALTERNATE NAMES: | AXIN2 Antibody: AXIL, ODCRCS, Axin-2, Axin-like protein, Axil |
| ACCESSION NO.: | AAF22799 |
| PROTEIN GI NO.: | 195927059 |
| GENE ID: | 8313 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. |
| REFERENCES: | 1) Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. Cancer Res.2007; 13:4042-5. | 2) Behrens J, Jerchow BA, Wurtele M, et al. Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta. Science1998; 280:596-9. | 3) Hadjihannas MV and Behrens J. CIN by WNT: growth pathways, mitotic control and chromosome instability in cancer. Cell Cycle2006; 5:2077-81. | 4) Mai M, Qian C, Yokomizo A, et al. Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24. Genomics1999; 55:341-4. |
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