Autophagy Protein Detection Set Cat. No.: PSI-1813

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psi-iconSpecifications
SPECIES REACTIVITY:Human
TESTED APPLICATIONS:IF, IHC, WB
APPLICATIONS:These polyclonal antibodies can be used for detection of APG7, Beclin-1, LAMP1, LAMP2 and PIST by immunoblot at 1 - 2 μg/mL. APG7, Beclin-1 and PIST antibodies can detect their respective proteins via immunohistochemistry at 1 - 10 μg/mL, and Immunoflourescence.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
IMMUNOGEN:Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
psi-iconSet Contents
Catalog Number 3615Catalog number 3615 — APG7 Antibody
Catalog Number 3611Catalog number 3611 — Beclin-1 Antibody
Catalog Number 3629Catalog number 3629 — LAMP-1 Antibody
Catalog Number 3627Catalog number 3627 — LAMP-2 Antibody
Catalog Number 3631Catalog number 3631 — PIST Antibody
psi-iconProperties
PHYSICAL STATE:Liquid
BUFFER:PBS containing 0.02% sodium azide.
CONCENTRATION:Antibody 1 mg/mL
STORAGE CONDITIONS:Stable at 4˚C for three months, store at -20˚C for up to one year.
psi-iconAdditional Info
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground
BACKGROUND:Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. PIST is a protein that interacts with Beclin-1 through its coiled-coil domain and can modulate Beclin-1 activity. LAMP1 and LAMP2 are homologous, highly glycosylated proteins associated with the lysosome and are thought to have overlapping functions. Mice lacking LAMP1 have very minor defects compared to those deficient in LAMP2 expression. However, the loss of both proteins results in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.

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