Autophagy protein 10 Recombinant Protein Cat. No.: 91-998

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:E. coli
SEQUENCE:Met1-Thr190
FUSION TAG:C-6 His, N-T7 tag
TESTED APPLICATIONS:
APPLICATIONS:This recombinant protein can be used for biological assays. For research use only.
psi-iconProperties
PURITY:Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
PREDICTED MOLECULAR WEIGHT:24.5 kD
PHYSICAL STATE:Liquid
BUFFER:Supplied as a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
STORAGE CONDITIONS:Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:Ubiquitin-Like-Conjugating Enzyme ATG10, Autophagy-Related Protein 10, APG10-Like, ATG10
ACCESSION NO.:Q9H0Y0
PROTEIN GI NO.:530379522
OFFICIAL SYMBOL:ATG10
GENE ID:83734
psi-iconBackground and References
BACKGROUND:Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.

FOR RESEARCH USE ONLY.

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