ATP11B Antibody Cat. No.: 5857

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (100%)
IMMUNOGEN: ATP11B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11B.

The immunogen is located within amino acids 290 - 340 of ATP11B.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: ATP11B antibody can be used for detection of ATP11B by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 17-004 - K-562 Cell Slide

psi-iconProperties
PURIFICATION:ATP11B Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ATP11B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATP11B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ATP11B
ALTERNATE NAMES:ATP11B Antibody: ATPIF, ATPIR, ATPIF, KIAA0956, Probable phospholipid-transporting ATPase IF, ATPase IR
ACCESSION NO.:NP_055431
PROTEIN GI NO.:62632750
GENE ID:23200
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ATP11B Antibody: ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.
REFERENCES:1) Mansharamani M, Hewetson A, and Chilton BS. Cloning and characterization of an atypical Type IV P-type ATPase that binds to the RING motif of RUSH transcription factors. J. Biol. Chem.2001; 276:3641-9.
2) Halleck MS, Lawler JF Jr, Blackshaw S, et al. Differential expression of putative transbilayer amphipath transporters. Physiol. Genomics1999; 1:139-50.
3) Zhang B, Groffen J, and Heisterkamp N. Resistance to farnesyltransferase inhibitors in Bcr/Abl-positive lymphoblastic leukemia by increased expression of a novel ABC transporter homolog ATP11a. Blood2005; 106:1355-61.

ANTIBODIES FOR RESEARCH USE ONLY.

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