ATOH8 Antibody Cat. No.: 5109

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: ATOH8 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATOH8.

The immunogen is located within the last 50 amino acids of ATOH8.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: ATOH8 antibody can be used for detection of ATOH8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 35 kDa

Observed: 40 kDa

psi-iconProperties
PURIFICATION:ATOH8 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ATOH8 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATOH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ATOH8
ALTERNATE NAMES:ATOH8 Antibody: HATH6, bHLHa21, ATH6, BHLHA21, Protein atonal homolog 8, Class A basic helix-loop-helix protein 21
ACCESSION NO.:NP_116216
PROTEIN GI NO.:226498192
GENE ID:84913
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ATOH8 Antibody: Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43-57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.
REFERENCES:1) Wasserman SM, Mehraban F, Komuves LG, et al. Gene expression profile of human endothelial cells exposed to sustained fluid shear stress. Physiol. Genomics 2002;12:13-23.
2) Ledent V and Vervoort M. The basic helix-loop-helix protein family: comparative genomics and phylogenetic analysis. Genome Res. 2001; 11:754-770.
3) Inoue C, Bae SK, Takatsuka K, et al. Math6, a bHLH gene expressed in the developing nervous system, regulates neuronal versus glial differentiation. Genes Cells 2001; 6:977-986.
4) Ross MD, Martinka S, Mukherjee A, et al. Math6 expression during kidney development and altered expression in a mouse model of glomerulosclerosis. Dev Dyn. 2006; 235:3102-3109.

ANTIBODIES FOR RESEARCH USE ONLY.

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