ATG7 Recombinant Protein Cat. No.: 95-122

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psi-iconSpecifications
SPECIES:Mouse
SOURCE SPECIES:E. coli
SEQUENCE:aa 450 - 698
FUSION TAG:Fusion Partner: C-terminal His-tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for WB and ELISA. For research use only.
BIOLOGICAL ACTIVITY: N/A
psi-iconProperties
PURITY:~95%
PREDICTED MOLECULAR WEIGHT:30 kDa (Calculated)
PHYSICAL STATE:Liquid
BUFFER:1X PBS containing 10% glycerol
STORAGE CONDITIONS:Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
psi-iconAdditional Info
ALTERNATE NAMES:ATG7 Recombinant Protein: Agp7, Apg7l, Atg7l, Gm21553, 1810013K23Rik
ACCESSION NO.:AAH58597
PROTEIN GI NO.:37589293
OFFICIAL SYMBOL:Atg7
GENE ID:74244
psi-iconBackground and References
BACKGROUND:Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).
REFERENCES:1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene . 2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4) Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.

FOR RESEARCH USE ONLY.

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