ATG5 Recombinant Protein Cat. No.: 95-121

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psi-iconSpecifications
SPECIES:Mouse
SOURCE SPECIES:E. coli
SEQUENCE:aa 1 - 275
FUSION TAG:Fusion Partner: C-terminal His-tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for WB and ELISA. For research use only.
BIOLOGICAL ACTIVITY: N/A
psi-iconProperties
PURITY:~95%
PREDICTED MOLECULAR WEIGHT:34 kDa (Calculated)
PHYSICAL STATE:Liquid
BUFFER:1X PBS containing 10% glycerol
STORAGE CONDITIONS:Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
psi-iconAdditional Info
ALTERNATE NAMES:ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
ACCESSION NO.:AAH02166
PROTEIN GI NO.:10285391
OFFICIAL SYMBOL:ATG5
GENE ID:9474
psi-iconBackground and References
BACKGROUND:Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy (4). This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells (5).
REFERENCES:1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene . 2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4) Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.

FOR RESEARCH USE ONLY.

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