ATG5 Antibody Cat. No.: 5031

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psi-iconSpecifications
HOST SPECIES:Chicken
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat (100%), Bovine: (94%), Pig: (94%)
IMMUNOGEN: Anti-ATG5 antibody (5031) was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.

The immunogen is located within the first 50 amino acids of ATG5.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: WB: 1-2 μg/mL; IHC-P: 2.5 μg/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.
POSITIVE CONTROL:1) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
 3) Cat. No. 1224 - Daudi Cell Lysate
 4) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 32kD

Observed: 55kD (ATG5-ATG12 conjugation)
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar ATG5 expression profile in human and mouse cell lines detected by two independent anti-ATG5 antibodies that recognize different epitopes, 5031 against N-terminus domain and 64-229 against human recombinant protein.  ATG5 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies.  

ISOFORMS: Human ATG5 has 2 isoforms, including isoform L (275aa, 32.4kD) and isoform S (197aa, 22.9kD. This antibody detects only human isoform L. Mouse ATG5 has 1 isoform (275aa, 32.4kD). Rat ATG5 has only one isoform identified so far (275aa, 32.4kD).

psi-iconProperties
PURIFICATION:ATG5 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATG5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ATG5
ALTERNATE NAMES:ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
ACCESSION NO.:EAW48415
PROTEIN GI NO.:119568800
GENE ID:9474
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.
REFERENCES:1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
4) Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem.2007; 282:37298-302.

ANTIBODIES FOR RESEARCH USE ONLY.

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