ATG5 Antibody [1358CT289.125.123] Cat. No.: 64-229

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig, Bovine
IMMUNOGEN: A synthetic peptide corresponding to a sequence in the middle region of human APG5L(82-97aa DRFDQFWAINRKLMEY), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
TESTED APPLICATIONS: WB
APPLICATIONS: For WB starting dilution is: 1:1000
PREDICTED MOLECULAR WEIGHT: Human=32,23 kDa
Mouse=32 kDa
Rat=25 kDa

psi-iconProperties
PURIFICATION:Immunogen affinity purified.
CLONALITY:Monoclonal
ISOTYPE:IgG1, k
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ATG5
ALTERNATE NAMES:ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE
ACCESSION NO.:Q9H1Y0
GENE ID:9474
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Involved in autophagy vesicles formation. Conjugation with ATG12 through an ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. HCV utilizes ATG5 as a proviral factor during the onset of viral infection. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures; as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
REFERENCES:1) Hammond E.M.,et al.FEBS Lett. 425:391-395(1998).
2) Chen Y.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
3) Bechtel S.,et al.BMC Genomics 8:399-399(2007).
4) Mungall A.J.,et al.Nature 425:805-811(2003).

ANTIBODIES FOR RESEARCH USE ONLY.

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