ATG10 Antibody Cat. No.: 4399

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: ATG10 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ATG10.

The immunogen is located within the last 50 amino acids of ATG10.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ATG10 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate

psi-iconProperties
PURIFICATION:ATG10 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ATG10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATG10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ATG10
ALTERNATE NAMES:ATG10 Antibody: APG10, APG10L, pp12616, PP12616, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10, APG10-like
ACCESSION NO.:EAW95884
PROTEIN GI NO.:119616290
GENE ID:83734
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ATG10 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
REFERENCES:1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
2) Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
3) Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
4) Nemoto T, Tanida I, Tanida-Miyake E, et al. The mouse APG10 homologue, an E2-like enzyme for APG12p conjugation, facilitates MAP-LC3 modification. J. Biol. Chem.2003; 278:39517-26.

ANTIBODIES FOR RESEARCH USE ONLY.

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