ATG10 Antibody Cat. No.: 30-958

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG10.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ATG10 antibody can be used for detection of ATG10 by ELISA at 1:312500. ATG10 antibody can be used for detection of ATG10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
PREDICTED MOLECULAR WEIGHT: 25 kDa

psi-iconProperties
PURIFICATION:Antibody is purified by peptide affinity chromatography method.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATG10 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:ATG10
ALTERNATE NAMES:ATG10, APG10, APG10L, DKFZP586I0418, FLJ13954, pp12616
ACCESSION NO.:NP_113670
PROTEIN GI NO.:18594496
GENE ID:83734
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form.Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).
REFERENCES:1) Criollo, A., (2007) Cell Death Differ. 14 (5), 1029-1039.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.