ARR3 Antibody Cat. No.: 7643

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Mouse, Rat
IMMUNOGEN: ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3.

The immunogen is located within the last 50 amino acids of ARR3.
TESTED APPLICATIONS: IF, IHC-P, WB
APPLICATIONS: ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 43 kDa

Observed: 47 kDa

psi-iconProperties
PURIFICATION:ARR3 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ARR3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:ARR3
ALTERNATE NAMES:ARR3 Antibody: ARRX, ARRX, CAR, Arrestin-C, Cone arrestin, C-arrestin
ACCESSION NO.:NP_004303
PROTEIN GI NO.:156071467
GENE ID:407
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).
REFERENCES:1) Murakami A, Yajima T, Sakuma H, et al. X-arrestin: a new retinal arrestin mapping to the X chromosome. FEBS Lett. 1993; 334:203-9.
2) Gurevich VV and Gurevich EV. The structural basis of arrestin-mediated regulation of G-protein-coupled receptors. Pharmacol. Ther. 2006; 110:465-502.
3) Song X, Coffa S, Fu H, et al. How does arrestin assemble MAPKs into a signaling complex? J. Biol. Chem. 2009; 284:685-95.
4) Seo J, Tsakem EL, Breitman M, et al. Identification of arrestin-3-specific residues necessary for JNK3 kinase activation. J. Biol. Chem. 2011; 286:27894-901.

ANTIBODIES FOR RESEARCH USE ONLY.

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