ARF6 Antibody Cat. No.: 7699

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
IMMUNOGEN: ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6.

The immunogen is located within the last 50 amino acids of ARF6.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: ARF6 antibody is human, mouse and rat reactive.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 19 kDa

Observed: 18 kDa

psi-iconProperties
PURIFICATION:ARF6 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ARF6 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ARF6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:ARF6
ALTERNATE NAMES:ARF6 Antibody: ADP-ribosylation factor 6
ACCESSION NO.:NP_001654
PROTEIN GI NO.:4502211
GENE ID:382
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2). The product of this gene is localized to the plasma membrane (3), and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4).
REFERENCES:1) Graves RA, Tontonoz P, and Spiegelman BM. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Mol. Cell Biol. 1992; 12:1202-8.
2) Moss J and Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem. 1999; 193:153-7.
3) Cavenagh MM, Whitney JA, Carroll K, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J. Biol. Chem. 1996; 271:21767-74.
4) Schweitzer JK, Sedgewick AE, and D’Souza-Schorey C. ARF6-mediated endocytic recycling impacts cell movement, cell division and lipid homestasis. Semin. Cell Dev. Biol. 2011; 22:39-47.

ANTIBODIES FOR RESEARCH USE ONLY.

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