Specifications
Properties
Additional Info
Background
Specifications
| SPECIES REACTIVITY: | Human, Mouse, Rat |
|---|---|
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | ARC antibody can be used for detection of ARC by Western blot at 1:500 dilution. An approximately 25 kDa band can be detected. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| POSITIVE CONTROL: | 1) Cat. No. 1201 - HeLa Cell Lysate |
| PREDICTED MOLECULAR WEIGHT: | 25 kDa |
| IMMUNOGEN: | ARC antibody was raised against a 17 amino acid peptide near the amino terminus of human ARC. The immunogen is located within the first 50 amino acids of ARC. |
| HOST SPECIES: | Rabbit |
Properties
| PURIFICATION: | ARC Antibody is affinity chromatography purified via peptide column. |
|---|---|
| PHYSICAL STATE: | Liquid |
| BUFFER: | ARC Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | batch dependent |
| STORAGE CONDITIONS: | ARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
Additional Info
| ALTERNATE NAMES: | ARC Antibody: ARC, FCM, MYP, NOP, NOP30, ARC, Nucleolar protein 3, Apoptosis repressor with CARD, Myp |
|---|---|
| ACCESSION NO.: | NP_003937 |
| PROTEIN GI NO.: | 4505419 |
| OFFICIAL SYMBOL: | NOL3 |
| GENE ID: | 8996 |
Background
| BACKGROUND: | ARC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK BCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue. |
|---|---|
| REFERENCES: | 1) Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc Natl Acad Sci USA 1998;95:5156-60 | 2) Stoss O, Schwaiger FW, Cooper TA, Stamm S. Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c. J Biol Chem 1999;274(16):10951-62 | 3) Geertman R, McMahon A, Sabban EL. Cloning and characterization of cDNAs for novel proteins with glutamic acid-proline dipeptide tandem repeats. Biochim Biophys Acta 1996;1306(2-3):147-52 (WD 0300) |
For Research Use Only
