AQP2 Antibody Cat. No.: 7621

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (95%)
IMMUNOGEN: AQP2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AQP2.

The immunogen is located within the last 50 amino acids of AQP2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 30 kDa

Observed: 28 kDa

psi-iconProperties
PURIFICATION:AQP2 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:AQP2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AQP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:AQP2
ALTERNATE NAMES:AQP2 Antibody: AQP-CD, WCH-CD, Aquaporin-2, ADH water channel, AQP-2
ACCESSION NO.:NP_000477
PROTEIN GI NO.:4502179
GENE ID:359
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).
REFERENCES:1) Denker BM, Smith BL, Kuhada FP, et al. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 1988; 263:15634-42.
2) Barile M, Pisitkun T, Yu MJ, et al. Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct. Mol. Cell Proteomics 2005; 4:1095-106.
3) Bockenhauer D and Bichet DG. Inherited secondary nephrogenic diabetes insipidus: concentrating on humans. Am. J. Physiol. Renal Physiol. 2013; 304:F1037-42.

ANTIBODIES FOR RESEARCH USE ONLY.

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