SPECIES: | Human, Mouse |
SOURCE SPECIES: | HEK293 cells |
SEQUENCE: | The extracellular domain of human APRIL (aa 98-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a DDDDK-tag. |
FUSION TAG: | DDDDK Tag |
TESTED APPLICATIONS: | |
APPLICATIONS: | This recombinant proteins is for research use only. |
BIOLOGICAL ACTIVITY: | Stimulates B cell proliferation. |
PURITY: | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
PHYSICAL STATE: | Lyophilized |
BUFFER: | Contains PBS + 0.5% Trehalose. Reconstitute with 100 μl sterile water. |
CONCENTRATION: | 0.1mg/ml after reconstitution. |
STORAGE CONDITIONS: | Stable for at least 6 months after receipt when stored at -20˚C. |
ALTERNATE NAMES: | ACRP30headless:APRIL, ACRP30headless:CD256, ACRP30headless:TNFSF13, ACRP30headless:A-proliferation-inducing Ligand: |
ACCESSION NO.: | O75888 |
PROTEIN GI NO.: | 4507599 |
OFFICIAL SYMBOL: | TNFSF13 |
GENE ID: | 8741 |
BACKGROUND: | APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRILâ„¢ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells. |
FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.