| SPECIES: | Human, Mouse |
| SOURCE SPECIES: | HEK293 cells |
| SEQUENCE: | The extracellular domain of human APRIL (aa 98-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a DDDDK-tag. |
| FUSION TAG: | DDDDK Tag |
| TESTED APPLICATIONS: | |
| APPLICATIONS: | This recombinant proteins is for research use only. |
| BIOLOGICAL ACTIVITY: | Stimulates B cell proliferation. |
Specifications| PURITY: | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Contains PBS + 0.5% Trehalose. Reconstitute with 100 μl sterile water. |
| CONCENTRATION: | 0.1mg/ml after reconstitution. |
| STORAGE CONDITIONS: | Stable for at least 6 months after receipt when stored at -20˚C. |
| ALTERNATE NAMES: | ACRP30headless:APRIL, ACRP30headless:CD256, ACRP30headless:TNFSF13, ACRP30headless:A-proliferation-inducing Ligand: |
| ACCESSION NO.: | O75888 |
| PROTEIN GI NO.: | 4507599 |
| OFFICIAL SYMBOL: | TNFSF13 |
| GENE ID: | 8741 |
| BACKGROUND: | APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells. |
FOR RESEARCH USE ONLY.
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