APRIL Antibody Cat. No.: XP-5103

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL (human APRIL).
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA:

To detect hAPRIL by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hAPRIL.

Western Blot:
To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate

psi-iconProperties
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:APRIL antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:TNFSF13
ALTERNATE NAMES:APRIL, CD256, TALL2, ZTNF2, TALL-2, TRDL-1, UNQ383/PRO715, APRIL, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand
ACCESSION NO.:O75888
PROTEIN GI NO.:13124605
GENE ID:8741
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:APRIL (A PRoliferation Inducing Ligand) a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. Members of the TNF superfamily regulate immune responses and induce apoptosis.

ANTIBODIES FOR RESEARCH USE ONLY.

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