| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B. The immunogen is located within amino acids 20 - 70 of APOBEC3B. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D. |
| POSITIVE CONTROL: | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
| 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 42 kDa Observed: 48 kDa |
Specifications| PURIFICATION: | APOBEC3B antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | APOBEC3B Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | APOBEC3B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | APOBEC3B |
| ALTERNATE NAMES: | APOBEC3B Antibody: A3B, ARP4, ARCD3, PHRBNL, APOBEC1L, bK150C2.2, DJ742C19.2Phorbolin-1-related protein, A3B |
| ACCESSION NO.: | NP_004891 |
| PROTEIN GI NO.: | 393715117 |
| GENE ID: | 9582 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers; it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4). |
| REFERENCES: | 1) Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96. | 2) Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5? restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53. | 3) Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15. | 4) Burns MB, Temiz NA, and Harris RS. Evidence for APOBEC3B mutagenesis in multiple human cancers. Nat. Genet. 2013; 45:977-83. |
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