APOBEC3B Antibody Cat. No.: 7725

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B.

The immunogen is located within amino acids 20 - 70 of APOBEC3B.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 42 kDa

Observed: 48 kDa

psi-iconProperties
PURIFICATION:APOBEC3B antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:APOBEC3B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:APOBEC3B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:APOBEC3B
ALTERNATE NAMES:APOBEC3B Antibody: A3B, ARP4, ARCD3, PHRBNL, APOBEC1L, bK150C2.2, DJ742C19.2Phorbolin-1-related protein, A3B
ACCESSION NO.:NP_004891
PROTEIN GI NO.:393715117
GENE ID:9582
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers; it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4).
REFERENCES:1) Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
2) Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5? restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
3) Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
4) Burns MB, Temiz NA, and Harris RS. Evidence for APOBEC3B mutagenesis in multiple human cancers. Nat. Genet. 2013; 45:977-83.

ANTIBODIES FOR RESEARCH USE ONLY.

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