| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse |
| IMMUNOGEN: | APC6 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6. The immunogen is located within the first 50 amino acids of APC6. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
| 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Specifications| PURIFICATION: | APC6 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | APC6 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | APC6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | CDC16 |
| ALTERNATE NAMES: | APC6 Antibody: APC6, CUT9, ANAPC6, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, APC6 |
| ACCESSION NO.: | NP_003894 |
| PROTEIN GI NO.: | 118402580 |
| GENE ID: | 8881 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | APC6 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein. |
| REFERENCES: | 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. | 2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9. | 3) Wang J, Dye BT, Rajashankar KR, et al. Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC structure. Nat. Struct. Mol. Biol.2009; 16:987-9. |
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