| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | APC4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4. The immunogen is located within amino acids 40 - 90 of APC4. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | APC4 antibody can be used for detection of APC4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
Specifications| PURIFICATION: | APC4 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | APC4 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | APC4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | ANAPC4 |
| ALTERNATE NAMES: | APC4 Antibody: APC4, APC4, Anaphase-promoting complex subunit 4, Cyclosome subunit 4 |
| ACCESSION NO.: | Q9UJX5 |
| PROTEIN GI NO.: | 205371737 |
| GENE ID: | 29945 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | APC4 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three. |
| REFERENCES: | 1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. | 2) Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9. | 3) Thronton BR, Ng TM, Matyskiela ME, et al. An architectural map of the anaphase-promoting complex. Genes Dev.2006; 20:449-60. |
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