Apaf1 Antibody Cat. No.: 2015

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Apaf1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1.

The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.

The immunogen is located within the last 50 amino acids of Apaf1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 12-701 - Human Skin Tissue Slide
PREDICTED MOLECULAR WEIGHT: 130 kDa

psi-iconProperties
PURIFICATION:Apaf1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Apaf1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:APAF1
ALTERNATE NAMES:Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic protease-activating factor 1
ACCESSION NO.:AAC51678
PROTEIN GI NO.:2330015
GENE ID:317
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
REFERENCES:1) Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
2) Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
3) Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
4) Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (WD1299)

ANTIBODIES FOR RESEARCH USE ONLY.

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