Apaf-1 Antibody [2E12] Cat. No.: 36-175

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psi-iconSpecifications
HOST SPECIES:Rat
SPECIES REACTIVITY: Human
IMMUNOGEN: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
TESTED APPLICATIONS: ELISA, ICC, IHC, IP, WB
APPLICATIONS: Immunohistochemistry: (paraffin sections, 10ug/ml) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al.; (2000)) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
SPECIFICITY: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

psi-iconProperties
PURIFICATION:>95% (SDS-PAGE)
CLONALITY:Monoclonal
ISOTYPE:IgG2a, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Liquid. In PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -20˚C.

psi-iconAdditional Info
OFFICIAL SYMBOL:APAF1
ALTERNATE NAMES:Apoptosis Protease-activating Factor 1
ACCESSION NO.:AAC51678
PROTEIN GI NO.:2330015
GENE ID:317
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

ANTIBODIES FOR RESEARCH USE ONLY.

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